WAS polyclonal antibody
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More Files
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Western Blot (Cell lysate)
WAS polyclonal antibody (Cat # PAB6757) staining (0.03 ug/mL) of U-937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of WAS.
Immunogen
A synthetic peptide corresponding to human WAS.
Sequence
C-SPADKKRSGKKKI
Host
Goat
Theoretical MW (kDa)
52.9
Reactivity
Human
Specificity
This antibody is expected to no cross-reactivity with N WASP (WASL).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
WAS polyclonal antibody (Cat # PAB6757) staining (0.03 ug/mL) of U-937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WAS
Entrez GeneID
7454Protein Accession#
NP_000368.1Gene Name
WAS
Gene Alias
IMD2, THC, WASP
Gene Description
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq
Other Designations
OTTHUMP00000032395|Wiskott-Aldrich syndrome protein|thrombocytopenia 1 (X-linked)
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin.
Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J.
The Journal of Cell Biology 2005 Jan; 168(3):441.
Application：IF, WB-Tr, Rat, MTLn3, MTC cells.
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Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin.
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