Goat polyclonal antibody raised against synthetic peptide of RARA.
A synthetic peptide corresponding to human RARA.
Theoretical MW (kDa):
50.8, 50.8, 39.7
NP_000955.1 (isfa) and NP_001028775.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001028775.1). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar.
Antigen affinity purification
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:8000) Western blot (1-3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
RARA polyclonal antibody (Cat # PAB6755) staining (0.03 ug/mL) of human brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM
OTTHUMP00000164454,OTTHUMP00000164456,Retinoic acid receptor, alpha polypeptide,nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form