RBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
RBP1 polyclonal antibody (Cat # PAB6754) (0.1 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RBP1 polyclonal antibody (Cat # PAB6754) (5 ug/mL) staining of paraffin embedded human pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RBP1.
Immunogen
A synthetic peptide corresponding to human RBP1.
Sequence
VEGVVCKQVFKKVQ
Host
Goat
Theoretical MW (kDa)
22.3
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RBP1 polyclonal antibody (Cat # PAB6754) (0.1 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RBP1 polyclonal antibody (Cat # PAB6754) (5 ug/mL) staining of paraffin embedded human pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RBP1
Entrez GeneID
5947Protein Accession#
NP_002890.1Gene Name
RBP1
Gene Alias
CRABP-I, CRBP, CRBP1, CRBPI, RBPC
Gene Description
retinol binding protein 1, cellular
Omim ID
180260Gene Ontology
HyperlinkGene Summary
This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
retinol-binding protein 1, cellular
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Interactome
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Disease
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Publication Reference
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Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.
Bilbija D, Haugen F, Sagave J, Baysa A, Bastani N, Levy FO, Sirsjö A, Blomhoff R, Valen G.
PLoS One 2012 Sep; 7(9):e44740.
Application：WB-Ti, Mouse, Mouse hearts.
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Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity.
Farias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R.
Journal of the National Cancer Institute 2005 Jan; 97(1):21.
Application：IF, WB, Human, MTSV1-7 cells.
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Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.
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