PDE5A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PDE5A polyclonal antibody (Cat # PAB6741) staining (0.3 ug/mL) of human lung lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing PDE5A and probed with PDE5A polyclonal antibody (Cat # PAB6741) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDE5A.
Immunogen
A synthetic peptide corresponding to human PDE5A.
Sequence
C-LINGESGQAKRN
Host
Goat
Theoretical MW (kDa)
100, 94.8, 93.6
Reactivity
Human
Specificity
This antibody is expected to recognize all 4 reported isoforms (as represented by NP_001074, NP_236914, NP_246273). NP_237223 has been retracted (280905)
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PDE5A polyclonal antibody (Cat # PAB6741) staining (0.3 ug/mL) of human lung lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing PDE5A and probed with PDE5A polyclonal antibody (Cat # PAB6741) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDE5A
Entrez GeneID
8654Protein Accession#
NP_001074;NP_237223;NP_246273;NP_236914Gene Name
PDE5A
Gene Alias
CGB-PDE, CN5A, PDE5, PDE5A1
Gene Description
phosphodiesterase 5A, cGMP-specific
Omim ID
603310Gene Ontology
HyperlinkGene Summary
This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase|phosphodiesterase 5A
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Interactome
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Pathway
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Disease
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Publication Reference
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Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa.
Morelli A, Filippi S, Mancina R, Luconi M, Vignozzi L, Marini M, Orlando C, Vannelli GB, Aversa A, Natali A, Forti G, Giorgi M, Jannini EA, Ledda F, Maggi M.
Endocrinology 2004 May; 145(5):2253.
Application：IHC-P, WB-Ti, Human, Rabbit, Human penile tissues, Rabbit corpora cavernosa.
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Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa.
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