TPI1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
TPI1 polyclonal antibody (Cat # PAB6740) staining (0.001µg/ml) HepG2 (1), HEK293 (2), HeLa (3) and Jurkat (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TPI1 polyclonal antibody (Cat # PAB6740) (5 ug/mL) staining of paraffin embedded Human Pancreas.Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TPI1 polyclonal antibody (Cat # PAB6740) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Flow Cytometry
TPI1 polyclonal antibody (Cat # PAB6740) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TPI1.
Immunogen
A synthetic peptide corresponding to C-terminus of human TPI1.
Sequence
C-LKPEFVDIINAKQ
Host
Goat
Theoretical MW (kDa)
26.7
Reactivity
Dog, Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunohistochemistry (2-5 ug/mL)
Flow Cytometry (10 ug/mL)
Western Blot (0.001-0.01 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TPI1 polyclonal antibody (Cat # PAB6740) staining (0.001µg/ml) HepG2 (1), HEK293 (2), HeLa (3) and Jurkat (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TPI1 polyclonal antibody (Cat # PAB6740) (5 ug/mL) staining of paraffin embedded Human Pancreas.Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TPI1 polyclonal antibody (Cat # PAB6740) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
TPI1 polyclonal antibody (Cat # PAB6740) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — TPI1
Entrez GeneID
7167Protein Accession#
NP_000356.1;NP_001152759.1Gene Name
TPI1
Gene Alias
MGC88108, TPI
Gene Description
triosephosphate isomerase 1
Omim ID
190450Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
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Publication Reference
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Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.
Honore B, Buus S, Claesson MH.
Proteome Science 2008 Jun; 6:18.
Application：WB, Mouse, Mouse thymocytes, SM5, SM7 tumour cell line.
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Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study.
Vorum H, Ostergaard M, Rice GE, Honore B, Bek T.
Proteome Science 2007 Feb; 5:5.
Application：WB-Ti, Human, Ocular tissue from a patient with Leber's Congenital Amaurosis.
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Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.
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