MAPK1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MAPK1.
Immunogen
A synthetic peptide corresponding to human MAPK1.
Sequence
CAAGPEMVRGQVF
Host
Goat
Theoretical MW (kDa)
41.4
Reactivity
Human
Specificity
This antibody is expected to recognize an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2/MAPK1 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MAPK1 polyclonal antibody (Cat # PAB6721) (0.5 ug/mL) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Protein Accession#
NP_002736;NP_620407Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Interactome
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Publication Reference
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EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes.
Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon DY, Hong JT.
Biochemical and Biophysical Research Communications 2004 Aug; 320(4):1087.
Application:WB, Rat, Rat astrocytes.
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EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes.
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