PADI4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PADI4.
Immunogen
A synthetic peptide corresponding to human PADI4.
Sequence
AQGTLIRVTPEQPC
Host
Goat
Theoretical MW (kDa)
74
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PADI4 polyclonal antibody (Cat # PAB6703) (0.3 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PADI4
Entrez GeneID
23569Protein Accession#
NP_0.36519Gene Name
PADI4
Gene Alias
PAD, PAD4, PADI5, PDI4, PDI5
Gene Description
peptidyl arginine deiminase, type IV
Gene Ontology
HyperlinkGene Summary
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq
Other Designations
HL-60 PAD|OTTHUMP00000002482|PADI-H protein|peptidyl arginine deiminase, type V|protein-arginine deiminase
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Interactome
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Disease
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Publication Reference
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Human PAD4 regulates histone arginine methylation levels via demethylimination.
Wang Y, Wysocka J, Sayegh J, Lee YH, Perlin JR, Leonelli L, Sonbuchner LS, McDonald CH, Cook RG, Dou Y, Roeder RG, Clarke S, Stallcup MR, Allis CD, Coonrod SA.
Science 2004 Sep; 306(5694):279.
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Human PAD4 regulates histone arginine methylation levels via demethylimination.
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