Goat polyclonal antibody raised against synthetic peptide of ADAM12.
A synthetic peptide corresponding to human ADAM12.
Theoretical MW (kDa):
This antibody is expected to recognize both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS.
Antigen affinity purification
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:64000) Western blot (0.1-0.3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
ADAM12 polyclonal antibody (Cat # PAB6697) staining (0.1 ug/mL) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. [provided by RefSeq
OTTHUMP00000020727,OTTHUMP00000046766,a disintegrin and metalloproteinase domain 12 (meltrin alpha),meltrin alpha