DOK5 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 overexpressing DOK5 and probed with DOK5 polyclonal antibody (Cat # PAB6689) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DOK5 polyclonal antibody (Cat # PAB6689) (1 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DOK5.
Immunogen
A synthetic peptide corresponding to human DOK5.
Sequence
CLHRTETFPAYRSEH
Host
Goat
Theoretical MW (kDa)
35.5
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms of DOK5.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 overexpressing DOK5 and probed with DOK5 polyclonal antibody (Cat # PAB6689) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DOK5 polyclonal antibody (Cat # PAB6689) (1 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DOK5
Entrez GeneID
55816Protein Accession#
NP_060901;NP_808874Gene Name
DOK5
Gene Alias
C20orf180, MGC16926
Gene Description
docking protein 5
Omim ID
608334Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. [provided by RefSeq
Other Designations
OTTHUMP00000031322|downstream of tyrosine kinase 5
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Interactome
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Disease
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Publication Reference
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Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5.
Cai D, Dhe-Paganon S, Melendez PA, Lee J, Shoelson SE.
The Journal of Biological Chemistry 2003 May; 278(28):25323.
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Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5.
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