TIMM50 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TIMM50.
Immunogen
A synthetic peptide corresponding to human TIMM50.
Sequence
CDVRTVLEHYALEDD
Host
Goat
Theoretical MW (kDa)
50.5
Reactivity
Human
Specificity
This antibody is expected to recognize an human TIM50.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.5-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TIMM50 polyclonal antibody (Cat # PAB6688) staining (0.5 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TIMM50
Entrez GeneID
92609Protein Accession#
NP_001001563Gene Name
TIMM50
Gene Alias
MGC102733, TIM50, TIM50L
Gene Description
translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
Omim ID
607381Gene Ontology
HyperlinkOther Designations
Tim50-like protein|homolog of yeast Tim50|translocase of inner mitochondrial membrane 50 homolog
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Interactome
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Publication Reference
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Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.
Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES.
The Journal of Biological Chemistry 2004 Jun; 279(23):24813.
Application:WB-Tr, Human, HEK 293T cells.
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Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.
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