FHL1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FHL1 polyclonal antibody (Cat # PAB6686) staining (0.05 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FHL1 polyclonal antibody (Cat # PAB6686, 2.5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FHL1.
Immunogen
A synthetic peptide corresponding to internal region of human FHL1.
Sequence
CNKRFVFHNEQVY
Host
Goat
Theoretical MW (kDa)
31.9
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize human FHL1 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (0.05-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.2-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FHL1 polyclonal antibody (Cat # PAB6686) staining (0.05 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FHL1 polyclonal antibody (Cat # PAB6686, 2.5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FHL1
Entrez GeneID
2273Protein Accession#
NP_001440.1Gene Name
FHL1
Gene Alias
FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA, bA535K18.1
Gene Description
four and a half LIM domains 1
Omim ID
300163Gene Ontology
HyperlinkGene Summary
LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM
Other Designations
Four-and-a-half LIM domains 1|OTTHUMP00000024097|bA535K18.1 (LIM protein SLIMMER)
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Interactome
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Publication Reference
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Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA.
The Journal of Biological Chemistry 1999 Sep; 274(38):27083.
Application：IF, WB, Human, Mouse, Human skeletal muscle, Sol8 cells.
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Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
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