EHD2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
EHD2 polyclonal antibody (Cat # PAB6684) staining (0.01 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing EHD2 and probed with EHD2 polyclonal antibody (Cat # PAB6684) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EHD2.
Immunogen
A synthetic peptide corresponding to C-terminus of human EHD2.
Sequence
CRLVPPSKRRHKGSA
Host
Goat
Theoretical MW (kDa)
61.2
Reactivity
Human
Specificity
This antibody is expected to recognize EHD1 protein as well as EHD2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EHD2 polyclonal antibody (Cat # PAB6684) staining (0.01 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing EHD2 and probed with EHD2 polyclonal antibody (Cat # PAB6684) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EHD2
Entrez GeneID
30846Protein Accession#
NP_055416Gene Name
EHD2
Gene Alias
FLJ96617, PAST2
Gene Description
EH-domain containing 2
Omim ID
605890Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq
Other Designations
EH domain containing 2
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Interactome
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Pathway
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Publication Reference
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Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes.
Guilherme A, Soriano NA, Furcinitti PS, Czech MP.
The Journal of Biological Chemistry 2004 Sep; 279(38):40062.
Application：IF, WB, Monkey, Mouse, 3T3-L1, COS-1 cells.
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Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes.
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