SCARF1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SCARF1 polyclonal antibody (Cat # PAB6682) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SCARF1.
Immunogen
A synthetic peptide corresponding to internal regoin of human SCARF1.
Sequence
SELDPKGQHVC
Host
Goat
Theoretical MW (kDa)
87.4
Reactivity
Human
Specificity
This antibody is expected to recognize all 5 isoforms of SCARF1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SCARF1 polyclonal antibody (Cat # PAB6682) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SCARF1
Entrez GeneID
8578Protein Accession#
NP_003684.2;NP_663325.1;NP_663327.2Gene Name
SCARF1
Gene Alias
KIAA0149, MGC47738, SREC
Gene Description
scavenger receptor class F, member 1
Omim ID
607873Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a scavenger receptor that is expressed in endothelial cells and mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Transcription factors ZNF444/EZF-2 and SP1 are found to be responsible for the regulation of this gene. Five alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
acetyl LDL receptor|scavenger receptor expressed by endothelial cells
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Interactome
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Disease
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Publication Reference
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Expression cloning of a novel scavenger receptor from human endothelial cells.
Adachi H, Tsujimoto M, Arai H, Inoue K.
The Journal of Biological Chemistry 1997 Dec; 272(50):31217.
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Expression cloning of a novel scavenger receptor from human endothelial cells.
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