CASP4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CASP4.
Immunogen
A synthetic peptide corresponding to human CASP4.
Sequence
CKERAEEIYPIKERN
Host
Goat
Theoretical MW (kDa)
43.3, 36.7
Reactivity
Human
Specificity
This antibody is expected to recognize all 3 isoforms of CASP4 (alpha, represented by NP_001216, gamma, NP-150649 and delta NP_150650).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CASP4 polyclonal antibody (Cat # PAB6681) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Tissue lysate)
CASP4 polyclonal antibody (Cat # PAB6681) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CASP4
Entrez GeneID
837Protein Accession#
NP_001216;NP_150649;NP_150650Gene Name
CASP4
Gene Alias
ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX
Gene Description
caspase 4, apoptosis-related cysteine peptidase
Omim ID
602664Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
apoptotic cysteine protease Mih1/TX|caspase 4|caspase 4, apoptosis-related cysteine protease
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Interactome
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Disease
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Publication Reference
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Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
Munday NA, Vaillancourt JP, Ali A, Casano FJ, Miller DK, Molineaux SM, Yamin TT, Yu VL, Nicholson DW.
The Journal of Biological Chemistry 1995 Jun; 270(26):15870.
Application:WB-Ce, WB-Ti, Human, Human tissues, THP-1 cells.
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Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
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