PPARD polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPARD.
Immunogen
A synthetic peptide corresponding to C-terminus of human PPARD.
Sequence
CHPLLQEIYKDMY
Host
Goat
Theoretical MW (kDa)
49.9
Reactivity
Human, Rat
Specificity
This antibody is expected to recognize isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_80.3184) with a different C-terminus.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PPARD polyclonal antibody (Cat # PAB6679) (0.3 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPARD
Entrez GeneID
5467Protein Accession#
NP_006229.1Gene Name
PPARD
Gene Alias
FAAR, MGC3931, NR1C2, NUC1, NUCI, NUCII, PPAR-beta, PPARB
Gene Description
peroxisome proliferator-activated receptor delta
Omim ID
600409Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. [provided by RefSeq
Other Designations
OTTHUMP00000016256|nuclear hormone receptor 1|peroxisome proliferative activated receptor, delta
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Interactome
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Pathway
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Disease
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Publication Reference
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Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells.
Park BH, Vogelstein B, Kinzler KW.
PNAS 2001 Feb; 98(5):2598.
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Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells.
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