SAMSN1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SAMSN1 polyclonal antibody (Cat # PAB6673) (1 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SAMSN1.
Immunogen
A synthetic peptide corresponding to human SAMSN1.
Sequence
IRLDTLSASLGRSC
Host
Goat
Theoretical MW (kDa)
41.7
Reactivity
Human
Specificity
The immunogen sequence is present in NP_071419.2 (and in AAK07746) but is no longer present in the latest reference sequence (NP_071419.3 at 05/10/05). This latest version has not been subject to final review by NCBI. This antibody may be recognising one particular isoform.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SAMSN1 polyclonal antibody (Cat # PAB6673) (1 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAMSN1
Entrez GeneID
64092Protein Accession#
NP_071419.2Gene Name
SAMSN1
Gene Alias
HACS1, NASH1, SASH2, SH3D6B
Gene Description
SAM domain, SH3 domain and nuclear localization signals 1
Omim ID
607978Gene Ontology
HyperlinkGene Summary
SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains (Claudio et al., 2001 [PubMed 11536050]).[supplied by OMIM
Other Designations
OTTHUMP00000083034|SAM and SH3 domain containing 2|SAM domain, SH3 domain and nuclear localisation signals, 1|SAM domain, SH3 domain and nuclear localization signals, 1|SH3-SAM adaptor protein|hematopoietic adaptor containing SH3 and SAM domains 1|nuclear
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Disease
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Publication Reference
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The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades.
Zhu YX, Benn S, Li ZH, Wei E, Masih-Khan E, Trieu Y, Bali M, McGlade CJ, Claudio JO, Stewart AK.
The Journal of Experimental Medicine 2004 Sep; 200(6):737.
Application：IF, IHC, IP, WB, Human, Mouse, Human BJAB cells, Human peripheral B cells, Lymph node, Spleen.
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The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades.
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