SH2B3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SH2B3 polyclonal antibody (Cat # PAB6669) (0.3 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing SH2B3 and probed with SH2B3 polyclonal antibody (Cat # PAB6669) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SH2B3.
Immunogen
A synthetic peptide corresponding to human SH2B3.
Sequence
NGPALQPSSPC
Host
Goat
Theoretical MW (kDa)
63.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SH2B3 polyclonal antibody (Cat # PAB6669) (0.3 ug/mL) staining of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SH2B3 and probed with SH2B3 polyclonal antibody (Cat # PAB6669) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SH2B3
Entrez GeneID
10019Protein Accession#
NP_005466.1Gene Name
SH2B3
Gene Alias
CELIAC13, LNK
Gene Description
SH2B adaptor protein 3
Omim ID
605093Gene Ontology
HyperlinkGene Summary
T-cell activation requires stimulation of the T-cell receptor (TCR; see MIM 186880)-CD3 (see CD3Z; MIM 186780) complex, followed by recruitment of an array of intracellular signaling proteins (e.g., GRB2 (MIM 108355) and PLCG1 (MIM 172420)). Mediating the interaction between the extracellular receptors and intracellular signaling pathways are adaptor proteins such as LAT (MIM 602354), TRIM (MIM 604962), and LNK.[supplied by OMIM
Other Designations
lymphocyte adaptor protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.
Li Y, He X, Schembri-King J, Jakes S, Hayashi J.
Journal of Immunology 2000 May; 164(10):5199.
Application：IP, WB-Tr, Human, Monkey, COS-7, Jurkat cells.
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Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation.
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