ACTA2 polyclonal antibody (Cat # PAB6668) staining (0.5 ug/mL) of human duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) over expressing human ACTA2 with DYKDDDDK tag probed with ACTA2 polyclonal antibody ( Cat # PAB6668) (1 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with ACTA2 polyclonal antibody ( Cat # PAB6668 (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq