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Last updated: 2017/10/15
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ACTA2 polyclonal antibody

  • Catalog # : PAB6668
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ACTA2.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human ACTA2.
  • Sequence:
  • EEEDSTALVC
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 42
  • Reactivity:
  • Human, Mouse, Pig, Rat
  • Specificity:
  • Variants NP_001604.1 and NP_001135417.1 encode the same protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:1000)
    Western blot (0.01-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ACTA2 polyclonal antibody (Cat # PAB6668) staining (0.5 ug/mL) of human duodenum lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 lysate (10 ug protein in RIPA buffer) over expressing human ACTA2 with DYKDDDDK tag probed with ACTA2 polyclonal antibody ( Cat # PAB6668) (1 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with ACTA2 polyclonal antibody ( Cat # PAB6668 (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 59
  • Protein Accession#:
  • NP_001604.1;NP_001135417.1
  • Gene Name:
  • ACTA2
  • Gene Alias:
  • AAT6,ACTSA
  • Gene Description:
  • actin, alpha 2, smooth muscle, aorta
  • Gene Summary:
  • The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020042,alpha 2 actin,alpha-cardiac actin,growth-inhibiting gene 46
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