XAGE1D polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of XAGE1D.
Immunogen
A synthetic peptide corresponding to C-terminus of human XAGE1D.
Sequence
CGFGFRRQGEDNT
Host
Goat
Theoretical MW (kDa)
8
Reactivity
Human
Specificity
This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
XAGE1D polyclonal antibody (Cat # PAB6659) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — XAGE1D
Entrez GeneID
9503Protein Accession#
NP_597673Gene Name
XAGE1D
Gene Alias
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Gene Description
X antigen family, member 1D
Omim ID
300289Gene Ontology
HyperlinkGene Summary
This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to the use of an alternative transcription start site and an alternative translation initiation codon, results in multiple variants that encode different isoforms. [provided by RefSeq
Other Designations
G antigen, family D, 2|OTTHUMP00000042367
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Publication Reference
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XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma.
Liu XF, Helman LJ, Yeung C, Bera TK, Lee B, Pastan I.
Cancer Research 2000 Sep; 60(17):4752.
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XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma.
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