RANBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RANBP1 polyclonal antibody (Cat # PAB6654) (0.3 ug/mL) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
RANBP1 polyclonal antibody (Cat # PAB6654) (0.03 ug/mL) staining of A431 (1), (0.5 ug/mL) HeLa (2) and (0.1 ug/mL) MCF7 (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with RANBP1 polyclonal antibody (Cat # PAB6654) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence
RANBP1 polyclonal antibody (Cat # PAB6654) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence
RANBP1 polyclonal antibody (Cat # PAB6654) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
RANBP1 polyclonal antibody (Cat # PAB6654) Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RANBP1.
Immunogen
A synthetic peptide corresponding to human RANBP1.
Sequence
C-LSVKEETKEDAEEKQ
Host
Goat
Theoretical MW (kDa)
23.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (3-5 ug/mL)
Western Blot (0.03-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RANBP1 polyclonal antibody (Cat # PAB6654) (0.3 ug/mL) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
RANBP1 polyclonal antibody (Cat # PAB6654) (0.03 ug/mL) staining of A431 (1), (0.5 ug/mL) HeLa (2) and (0.1 ug/mL) MCF7 (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with RANBP1 polyclonal antibody (Cat # PAB6654) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
RANBP1 polyclonal antibody (Cat # PAB6654) Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
RANBP1 polyclonal antibody (Cat # PAB6654) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
RANBP1 polyclonal antibody (Cat # PAB6654) Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — RANBP1
Entrez GeneID
5902Protein Accession#
NP_002873Gene Name
RANBP1
Gene Alias
HTF9A, MGC88701
Gene Description
RAN binding protein 1
Omim ID
601180Gene Ontology
HyperlinkGene Summary
Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
Bischoff FR, Krebber H, Smirnova E, Dong W, Ponstingl H.
The EMBO Journal 1995 Feb; 14(4):705.
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Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.
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