Lime1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Lime1.
Immunogen
A synthetic peptide corresponding to mouse Lime1.
Sequence
C-ENVYESIKEMGL
Host
Goat
Theoretical MW (kDa)
29.6
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Lime1 polyclonal antibody (Cat # PAB6643) staining (1 ug/mL) of mouse heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Lime1
Entrez GeneID
72699Protein Accession#
NP_076173.1Gene Name
Lime1
Gene Alias
2810038K19Rik, LIME
Gene Description
Lck interacting transmembrane adaptor 1
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000018005
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Publication Reference
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LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation.
Hur EM, Son M, Lee OH, Choi YB, Park C, Lee H, Yun Y.
The Journal of Experimental Medicine 2003 Nov; 198(10):1463.
Application:IF, WB, Human, Jurkat cells.
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LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation.
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