DEF6 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DEF6 polyclonal antibody (Cat # PAB6625) (1 ug/mL) staining of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DEF6.
Immunogen
A synthetic peptide corresponding to human DEF6.
Sequence
ALRKELLKSIWYA-C
Host
Goat
Theoretical MW (kDa)
73.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DEF6 polyclonal antibody (Cat # PAB6625) (1 ug/mL) staining of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DEF6
Entrez GeneID
50619Protein Accession#
NP_071330Gene Name
DEF6
Gene Alias
IBP, SLAT
Gene Description
differentially expressed in FDCP 6 homolog (mouse)
Omim ID
610094Gene Ontology
HyperlinkGene Summary
DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM
Other Designations
IRF4-binding protein|OTTHUMP00000016251|differentially expressed in FDCP 6 homolog
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Interactome
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Disease
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Publication Reference
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SWAP-70-like adapter of T cells, an adapter protein that regulates early TCR-initiated signaling in Th2 lineage cells.
Tanaka Y, Bi K, Kitamura R, Hong S, Altman Y, Matsumoto A, Tabata H, Lebedeva S, Bushway PJ, Altman A.
Immunity 2003 Mar; 18(3):403.
Application：IF, WB-Ce, WB-Ti, WB-Tr, Human, Mouse, Human T cells, TCR/KAE, AD10, Jurkat, EL4, CH27, THP-1 cells, Brain, Heart, Lung, Liver, Kidney, Thymus, Spleen, LN, BM, PBL, Muscle, Testis.
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SWAP-70-like adapter of T cells, an adapter protein that regulates early TCR-initiated signaling in Th2 lineage cells.
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