ACTR1B polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACTR1B.
Immunogen
A synthetic peptide corresponding to human ACTR1B.
Sequence
C-YEEDGSRAIHRKT
Host
Goat
Theoretical MW (kDa)
42.3
Reactivity
Human
Specificity
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACTR1B polyclonal antibody (Cat # PAB6618) staining (1 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACTR1B polyclonal antibody (Cat # PAB6618, 3.8 ug/mL) staining of paraffin embedded human uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTR1B
Entrez GeneID
10120Protein Accession#
NP_005726Gene Name
ACTR1B
Gene Alias
ARP1B, CTRN2, PC3
Gene Description
ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Omim ID
605144Gene Ontology
HyperlinkGene Summary
This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq
Other Designations
ARP1 actin-related protein 1 homolog B, centractin beta|centractin beta
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Interactome
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Publication Reference
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Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.
Clark SW, Staub O, Clark IB, Holzbaur EL, Paschal BM, Vallee RB, Meyer DI.
Molecular Biology of the Cell 1994 Dec; 5(12):1301.
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Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.
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