Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chio A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Singleton A, Hardy J, Traynor BJ.BMC Neurol. 2006 Dec 13;6:44.
Western Blot (Cell lysate)
IFT74 polyclonal antibody (Cat # PAB6611) staining (0.3 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IFT74 polyclonal antibody (Cat # PAB6611, 5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data were kindly provided by Dr. Bryan Traynor.
A) IFT74 polyclonal antibody (Cat # PAB6611) (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. See Application Notes!)