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Last updated: 2017/2/26
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IFT74 polyclonal antibody

  • Catalog # : PAB6611
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of IFT74.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human IFT74.
  • Sequence:
  • C-KTIVDALHSTSGN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 69.2, 51.4
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 1.
  • Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.
    Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chio A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Singleton A, Hardy J, Traynor BJ.BMC Neurol. 2006 Dec 13;6:44.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • IFT74 polyclonal antibody (Cat # PAB6611) staining (0.3 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • IFT74 polyclonal antibody (Cat # PAB6611, 5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data were kindly provided by Dr. Bryan Traynor.
  • Immunofluorescence
  • Immunofluorescence
  • A) IFT74 polyclonal antibody (Cat # PAB6611) (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. See Application Notes!)
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_079379.1;AAK77221.1
  • Gene Name:
  • IFT74
  • Gene Alias:
  • CCDC2,CMG-1,CMG1,FLJ22621,MGC111562
  • Gene Description:
  • intraflagellar transport 74 homolog (Chlamydomonas)
  • Other Designations:
  • OTTHUMP00000022772,capillary morphogenesis protein 1,coiled-coil domain containing 2
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