AKAP8 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AKAP8.
Immunogen
A synthetic peptide corresponding to human AKAP8.
Sequence
DQGYGGYGAWSAG-C
Host
Goat
Theoretical MW (kDa)
76
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AKAP8 polyclonal antibody (Cat # PAB6604) staining (1 ug/mL) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKAP8
Entrez GeneID
10270Protein Accession#
NP_005849Gene Name
AKAP8
Gene Alias
AKAP95, DKFZp586B1222
Gene Description
A kinase (PRKA) anchor protein 8
Omim ID
604692Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA. [provided by RefSeq
Other Designations
A-kinase anchor protein 8|A-kinase anchor protein, 95kDa
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Interactome
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Publication Reference
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Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95.
Eide T, Coghlan V, Orstavik S, Holsve C, Solberg R, Skalhegg BS, Lamb NJ, Langeberg L, Fernandez A, Scott JD, Jahnsen T, Tasken K.
Experimental Cell Research 1998 Feb; 238(2):305.
Application:IF, WB-Ce, Human, Hs-68 fibroblasts, HeLa cells.
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Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95.
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