SMUG1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMUG1.
Immunogen
A synthetic peptide corresponding to human SMUG1.
Sequence
PQAFLLGSIHEPA-C
Host
Goat
Theoretical MW (kDa)
29.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SMUG1 polyclonal antibody (Cat # PAB6601) staining (0.5 ug/mL) of MOLT 4 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMUG1
Entrez GeneID
23583Protein Accession#
NP_055126Gene Name
SMUG1
Gene Alias
FDG, HMUDG, MGC104370, UNG3
Gene Description
single-strand-selective monofunctional uracil-DNA glycosylase 1
Omim ID
607753Gene Ontology
HyperlinkGene Summary
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair.[supplied by OMIM
Other Designations
single-strand selective monofunctional uracil DNA glycosylase
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Interactome
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Pathway
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Disease
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Publication Reference
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Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1.
Boorstein RJ, Cummings A Jr, Marenstein DR, Chan MK, Ma Y, Neubert TA, Brown SM, Teebor GW.
The Journal of Biological Chemistry 2001 Nov; 276(45):41991.
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Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1.
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