AKAP3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
AKAP3 polyclonal antibody (Cat # PAB6598) (1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AKAP3.
Immunogen
A synthetic peptide corresponding to human AKAP3.
Sequence
C-TPLQLLDWLMVNL
Host
Goat
Theoretical MW (kDa)
94.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AKAP3 polyclonal antibody (Cat # PAB6598) (1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKAP3
Entrez GeneID
10566Protein Accession#
NP_006413Gene Name
AKAP3
Gene Alias
AKAP110, FSP95, PRKA3, SOB1
Gene Description
A kinase (PRKA) anchor protein 3
Omim ID
604689Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family, and is expressed in testis only. The encoded protein contains an RII-binding domain, and is predicted to participate in protein-protein interactions with the R-subunit of the PKA. This protein is localized to the ribs of the fibrous sheath in the principal piece of the sperm tail. It may function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. [provided by RefSeq
Other Designations
A-kinase anchor protein 3|A-kinase anchor protein, 110kDa|Fibrous Sheath Protein of 95 kDa|fibrous sheath protein, 95kDa|fibrousheathin 1|protein kinase A anchoring protein 3|sperm oocyte-binding protein 1
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Interactome
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Publication Reference
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Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition.
Lefevre A, Duquenne C, Rousseau-Merck MF, Rogier E, Finaz C.
Biochemical and Biophysical Research Communications 1999 May; 259(1):60.
Application：WB, Human, Human spermatozoa.
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Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition.
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