SIRT4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SIRT4 polyclonal antibody (Cat # PAB6590) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing SIRT4 and probed with SIRT4 polyclonal antibody (Cat # PAB6590) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SIRT4 polyclonal antibody (Cat # PAB6590) (2.5 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIRT4.
Immunogen
A synthetic peptide corresponding to C-terminus of human SIRT4.
Sequence
SRCGELLPLIDPC
Host
Goat
Theoretical MW (kDa)
35.2
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SIRT4 polyclonal antibody (Cat # PAB6590) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SIRT4 and probed with SIRT4 polyclonal antibody (Cat # PAB6590) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SIRT4 polyclonal antibody (Cat # PAB6590) (2.5 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIRT4
Entrez GeneID
23409Protein Accession#
NP_036372.1Gene Name
SIRT4
Gene Alias
MGC130046, MGC130047, MGC57437, SIR2L4
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Omim ID
604482Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq
Other Designations
sir2-like 4|sirtuin 4|sirtuin type 4
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Interactome
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Disease
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Publication Reference
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Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.
Frye RA.
Biochemical and Biophysical Research Communications 1999 Jun; 260(1):273.
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Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.
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