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Last updated: 2017/1/22
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SIRT4 polyclonal antibody

  • Catalog # : PAB6590
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SIRT4.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human SIRT4.
  • Sequence:
  • SRCGELLPLIDPC
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 35.2
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • SIRT4 polyclonal antibody (Cat # PAB6590) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing SIRT4 and probed with SIRT4 polyclonal antibody (Cat # PAB6590) (mock transfection in first lane), tested by Origene.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • SIRT4 polyclonal antibody (Cat # PAB6590) (2.5 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • SIRT4
  • Gene Alias:
  • MGC130046,MGC130047,MGC57437,SIR2L4
  • Gene Description:
  • sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq
  • Other Designations:
  • sir2-like 4,sirtuin 4,sirtuin type 4
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