TAGLN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
TAGLN polyclonal antibody (Cat # PAB6587)(0.01 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TAGLN.
Immunogen
A synthetic peptide corresponding to C-terminus of human TAGLN.
Sequence
C-MTGYGRPRQIIS
Host
Goat
Theoretical MW (kDa)
22.6
Reactivity
Human, Mouse, Rabbit, Rat
Specificity
NP_003177.2 and NP_001001522.1 represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TAGLN polyclonal antibody (Cat # PAB6587)(0.01 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TAGLN
Entrez GeneID
6876Protein Accession#
NP_003177.2Gene Name
TAGLN
Gene Alias
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Gene Description
transgelin
Omim ID
600818Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SM22-alpha|transgelin variant 2
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Interactome
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Disease
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Publication Reference
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Proteomic profiling of chronic low-frequency stimulated fast muscle.
Donoghue P, Doran P, Wynne K, Pedersen K, Dunn MJ, Ohlendieck K.
Proteomics 2007 Sep; 7(18):3417.
Application：WB-Ti, Rabbit, Rabbit tibialis anterior muscles.
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Proteomic profiling of chronic low-frequency stimulated fast muscle.
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