TCP1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
TCP1 polyclonal antibody (Cat # PAB6585) staining (1 ug/mL) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TCP1 polyclonal antibody (Cat # PAB6585) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TCP1.
Immunogen
A synthetic peptide corresponding to human TCP1.
Sequence
C-SYEDAVHSGALND
Host
Goat
Theoretical MW (kDa)
60.3, 43.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
Immunohistochemistry (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
TCP1 polyclonal antibody (Cat # PAB6585) staining (1 ug/mL) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TCP1 polyclonal antibody (Cat # PAB6585) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — TCP1
Entrez GeneID
6950Protein Accession#
NP_110379.2Gene Name
TCP1
Gene Alias
CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha
Gene Description
t-complex 1
Omim ID
186980Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000017529|T-complex protein 1|T-complex protein 1, alpha subunit|tailless complex polypeptide 1
-
Interactome
-
Disease
-
Publication Reference
-
TCP1 complex is a molecular chaperone in tubulin biogenesis.
Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H.
Nature 1992 Jul; 358(6383):245.
Application：AFC, WB-Re, WB-Ti, Mouse, Mouse testes, Purified proteins.
-
TCP1 complex is a molecular chaperone in tubulin biogenesis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com