VPS28 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
VPS28 polyclonal antibody (Cat # PAB6584) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS28 polyclonal antibody (Cat # PAB6584) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS28.
Immunogen
A synthetic peptide corresponding to human VPS28.
Sequence
C-ESAYNAFNRFLHA
Host
Goat
Theoretical MW (kDa)
25.4
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_057292.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VPS28 polyclonal antibody (Cat # PAB6584) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS28 polyclonal antibody (Cat # PAB6584) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS28
Entrez GeneID
51160Protein Accession#
NP_057292.1Gene Name
VPS28
Gene Alias
MGC60323
Gene Description
vacuolar protein sorting 28 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq
Other Designations
vacuolar protein sorting 28|yeast class E protein Vps28p homolog
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Interactome
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Pathway
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Publication Reference
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TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
Bishop N, Woodman P.
The Journal of Biological Chemistry 2001 Apr; 276(15):11735.
Application：IF, WB-Ce, Monkey, Rat, COS, NRK cells.
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TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
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