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Last updated: 2017/2/19
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VPS28 polyclonal antibody

  • Catalog # : PAB6584
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of VPS28.
  • Immunogen:
  • A synthetic peptide corresponding to human VPS28.
  • Sequence:
  • C-ESAYNAFNRFLHA
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 25.4
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform 1 (NP_057292.1) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1-3 ug/mL)
    Immunohistochemistry (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • VPS28 polyclonal antibody (Cat # PAB6584) staining (1 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • VPS28 polyclonal antibody (Cat # PAB6584) (10 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • VPS28
  • Gene Alias:
  • MGC60323
  • Gene Description:
  • vacuolar protein sorting 28 homolog (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq
  • Other Designations:
  • vacuolar protein sorting 28,yeast class E protein Vps28p homolog
  • Gene Pathway
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