SH3BP1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SH3BP1 polyclonal antibody (Cat # PAB6583) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SH3BP1 polyclonal antibody (Cat # PAB6583) (0.3 ug/mL) staining of paraffin embedded human kidney. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SH3BP1.
Immunogen
A synthetic peptide corresponding to human SH3BP1.
Sequence
C-PQPRPRSLASETN
Host
Goat
Theoretical MW (kDa)
75.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SH3BP1 polyclonal antibody (Cat # PAB6583) staining (1 ug/mL) of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SH3BP1 polyclonal antibody (Cat # PAB6583) (0.3 ug/mL) staining of paraffin embedded human kidney. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SH3BP1
Entrez GeneID
23616Protein Accession#
NP_061830.3Gene Name
SH3BP1
Gene Alias
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Gene Description
SH3-domain binding protein 1
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000028981|SH3 domain binding protein 1
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Interactome
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Publication Reference
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RNA and protein interactors with TDP-43 in human spinal cord lysates in ALS.
Volkening K, Keller B, Leysta-Lantz C, Strong MJ.
Journal of Proteome Research 2018 Apr; 17(4):1712.
Application：WB, Human, HEK293T cells, lumbar spinal cord lysates.
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The DNA sequence of human chromosome 22.
Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al..
Nature 1999 Dec; 402(6761):489.
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RNA and protein interactors with TDP-43 in human spinal cord lysates in ALS.
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