VPS29 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
VPS29 polyclonal antibody (Cat # PAB6574) staining (0.1 ug/mL) of human spleen lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS29.
Immunogen
A synthetic peptide corresponding to human VPS29.
Sequence
C-DDVKVERIEYKKP
Host
Goat
Theoretical MW (kDa)
20.5, 20.9
Reactivity
Human
Specificity
This antibody is expected to recognize both human isoforms (represented by NP_057310.1; NP_476528.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VPS29 polyclonal antibody (Cat # PAB6574) staining (0.1 ug/mL) of human spleen lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS29
Entrez GeneID
51699Protein Accession#
NP_057310.1;NP_476528.1Gene Name
VPS29
Gene Alias
DC15, DC7, DKFZp564F0223, FLJ20492, PEP11
Gene Description
vacuolar protein sorting 29 homolog (S. cerevisiae)
Omim ID
606932Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq
Other Designations
retromer protein|vacuolar protein sorting 29|vacuolar sorting protein VPS29/PEP11|x 007 protein
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Interactome
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Publication Reference
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Molecular Basis of Insulin-Responsive GLUT4 Trafficking Systems Revealed by Single Molecule Imaging.
Hatakeyama H, Kanzaki M.
Traffic 2011 Dec; 12(12):1805.
Application：WB-Tr, Mouse, 3T3L1 Fibroblasts.
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Molecular Basis of Insulin-Responsive GLUT4 Trafficking Systems Revealed by Single Molecule Imaging.
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