VPS26A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
VPS26A polyclonal antibody (Cat # PAB6573) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing VPS26A and probed with VPS26A polyclonal antibody (Cat # PAB6573) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS26A.
Immunogen
A synthetic peptide corresponding to human VPS26A.
Sequence
C-SPESQASAEQPEM
Host
Goat
Theoretical MW (kDa)
38.2
Reactivity
Human
Specificity
This antibody is expected tp recognize isoform 1 (NP_004887.2) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VPS26A polyclonal antibody (Cat # PAB6573) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing VPS26A and probed with VPS26A polyclonal antibody (Cat # PAB6573) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS26A
Entrez GeneID
9559Protein Accession#
NP_004887.2Gene Name
VPS26A
Gene Alias
FLJ12930, HB58, Hbeta58, PEP8A, VPS26
Gene Description
vacuolar protein sorting 26 homolog A (S. pombe)
Omim ID
605506Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000019721|vacuolar protein sorting 26 A
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Interactome
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Disease
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