RANGAP1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RANGAP1.
Immunogen
A synthetic peptide corresponding to human RANGAP1.
Sequence
ASEDIAKLAETLAK-C
Host
Goat
Theoretical MW (kDa)
63.5
Reactivity
Human, Mouse
Specificity
In mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.1-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RANGAP1 polyclonal antibody (Cat # PAB6571) staining (0.2 ug/mL) of NIH/3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RANGAP1 polyclonal antibody (Cat # PAB6571) (2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RANGAP1
Entrez GeneID
5905Protein Accession#
NP_002874Gene Name
RANGAP1
Gene Alias
Fug1, KIAA1835, MGC20266, SD
Gene Description
Ran GTPase activating protein 1
Omim ID
602362Gene Ontology
HyperlinkGene Summary
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq
Other Designations
OTTHUMP00000028918|segregation distorter homolog|segregation distortion
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Interactome
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Publication Reference
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Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
Bischoff FR, Krebber H, Kempf T, Hermes I, Ponstingl H.
PNAS 1995 Feb; 92(5):1749.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.
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