KPNA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
KPNA2 polyclonal antibody (Cat # PAB6558) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunofluorescence
KPNA2 polyclonal antibody (Cat # PAB6558) staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red) . Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KPNA2.
Immunogen
A synthetic peptide corresponding to human KPNA2.
Sequence
C-QVQDGAPGTFNF
Host
Goat
Theoretical MW (kDa)
57.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
KPNA2 polyclonal antibody (Cat # PAB6558) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunofluorescence
KPNA2 polyclonal antibody (Cat # PAB6558) staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red) . Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KPNA2
Entrez GeneID
3838Protein Accession#
NP_002257.1Gene Name
KPNA2
Gene Alias
IPOA1, QIP2, RCH1, SRP1alpha
Gene Description
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Omim ID
600685Gene Ontology
HyperlinkGene Summary
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq
Other Designations
RAG cohort 1|importin alpha 1|importin alpha 2|importin-alpha-P1|karyopherin alpha 2|pendulin
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Interactome
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Disease
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Publication Reference
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Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein.
Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA.
PNAS 1994 Jun; 91(13):6156.
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Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein.
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