NUP85 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
NUP85 polyclonal antibody (Cat # PAB6540) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NUP85.
Immunogen
A synthetic peptide corresponding to C-terminus of human NUP85.
Sequence
C-ARAIIREGSLEGS
Host
Goat
Theoretical MW (kDa)
75
Reactivity
Human
Specificity
In contrast to other anti-Pericentrin antibodies available, this antibody is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75 KDa band (the expected size according to NP_079120) and not a 220 KDa band (as reported for other anti-pericentrin antibodies).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NUP85 polyclonal antibody (Cat # PAB6540) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUP85
Entrez GeneID
79902Protein Accession#
NP_079120.1Gene Name
NUP85
Gene Alias
FLJ12549, Nup75
Gene Description
nucleoporin 85kDa
Omim ID
170285Gene Ontology
HyperlinkGene Summary
Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160. [provided by RefSeq
Other Designations
frount|nucleoporin 85|nucleoporin Nup75
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Interactome
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Disease
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Publication Reference
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Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
Doxsey SJ, Stein P, Evans L, Calarco PD, Kirschner M.
Cell 1994 Feb; 76(4):639.
Application：IEM, IF, WB-Ce, Mouse, Rat, Xenopus, NIH/3T3, CHO, Rat-l cells, Embryos.
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Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
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