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Last updated: 2016/11/27
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NUP85 polyclonal antibody

  • Catalog # : PAB6540
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NUP85.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human NUP85.
  • Sequence:
  • C-ARAIIREGSLEGS
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 75
  • Reactivity:
  • Human
  • Specificity:
  • In contrast to other anti-Pericentrin antibodies available, this antibody is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75 KDa band (the expected size according to NP_079120) and not a 220 KDa band (as reported for other anti-pericentrin antibodies).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • NUP85 polyclonal antibody (Cat # PAB6540) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • NUP85
  • Gene Alias:
  • FLJ12549,Nup75
  • Gene Description:
  • nucleoporin 85kDa
  • Gene Summary:
  • Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160. [provided by RefSeq
  • Other Designations:
  • frount,nucleoporin 85,nucleoporin Nup75
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