GIPC1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GIPC1.
Immunogen
A synthetic peptide corresponding to human GIPC1.
Sequence
C-GAIGDAKVGRY
Host
Goat
Theoretical MW (kDa)
36, 26.1
Reactivity
Human
Specificity
This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GIPC1 polyclonal antibody (Cat # PAB6538) staining (0.03 ug/mL) of human HeLa cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GIPC1
Entrez GeneID
10755Protein Accession#
NP_005707.1;NP_974197.1;NP_974199.1;NP_974196.1;NP_974198.1;NP_974223.1Gene Name
GIPC1
Gene Alias
C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, NIP, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, TIP-2
Gene Description
GIPC PDZ domain containing family, member 1
Omim ID
605072Gene Ontology
HyperlinkGene Summary
GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]).[supplied by OMIM
Other Designations
GLUT1 C-terminal binding protein|IGF-1 receptor interacting protein 1|regulator of G-protein signalling 19 interacting protein 1
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Interactome
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Disease
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Publication Reference
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GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.
De Vries L, Lou X, Zhao G, Zheng B, Farquhar MG.
PNAS 1998 Oct; 95(21):12340.
Application:IF, WB-Ce, Human, HeLa cells.
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GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.
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