PLEK polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PLEK.
Immunogen
A synthetic peptide corresponding to human PLEK.
Sequence
C-EWIKAIQMASRTGK
Host
Goat
Theoretical MW (kDa)
40.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.05-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PLEK polyclonal antibody (Cat # PAB6527) staining (0.05 ug/mL) of human PBMCs lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLEK
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Interactome
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Disease
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Publication Reference
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Molecular cloning and expression of the major protein kinase C substrate of platelets.
Tyers M, Rachubinski RA, Stewart MI, Varrichio AM, Shorr RG, Haslam RJ, Harley CB.
Nature 1988 Jun; 333(6172):470.
Application:WB-Re, Recombinant proteins.
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Molecular cloning and expression of the major protein kinase C substrate of platelets.
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