PHLDA3 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (Cat # PAB6526) (1 ug/mL) overexpressing human PHLDA3 with C-terminal MYC tag probed (10 ug protein in RIPA buffer) with in lane 1 and probed with anti-MYC Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with PAB6526 (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PHLDA3.
Immunogen
A synthetic peptide corresponding to human PHLDA3.
Sequence
C-RARQSLGTGTLVS
Host
Goat
Theoretical MW (kDa)
13.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (Cat # PAB6526) (1 ug/mL) overexpressing human PHLDA3 with C-terminal MYC tag probed (10 ug protein in RIPA buffer) with in lane 1 and probed with anti-MYC Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with PAB6526 (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PHLDA3
Entrez GeneID
23612Protein Accession#
NP_0.36528Gene Name
PHLDA3
Gene Alias
TIH1
Gene Description
pleckstrin homology-like domain, family A, member 3
Omim ID
607054Gene Ontology
HyperlinkGene Summary
family A
Other Designations
OTTHUMP00000033919|OTTHUMP00000033920|pleckstrin homology-like domain, family A, member 2
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Interactome
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Publication Reference
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A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting.
Frank D, Mendelsohn CL, Ciccone E, Svensson K, Ohlsson R, Tycko B.
Mammalian Genome 1999 Dec; 10(12):1150.
Application：WB-Ti, Mouse, Adult mouse tissues.
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A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting.
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