OSBPL7 polyclonal antibody
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More Files
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Immunofluorescence
OSBPL7 polyclonal antibody (Cat # PAB6519) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of OSBPL7.
Immunogen
A synthetic peptide corresponding to human OSBPL7.
Sequence
C-EPGYGNMDGAVLW
Host
Goat
Theoretical MW (kDa)
95.4
Specificity
This antibody is expected to recognize both reported isoforms (represented by NP_060201and NP_665741)
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunofluorescence (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
OSBPL7 polyclonal antibody (Cat # PAB6519) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — OSBPL7
Entrez GeneID
114881Protein Accession#
NP_060201;NP_665741Gene Name
OSBPL7
Gene Alias
MGC71150, ORP7
Gene Description
oxysterol binding protein-like 7
Omim ID
606735Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding the same isoform have been identified. [provided by RefSeq
Other Designations
OSBP-related protein 7|oxysterol-binding protein-like protein 7|oxysterol-binding protein-related protein 7
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Interactome
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Disease
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