OSBPL5 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
OSBPL5 polyclonal antibody (Cat # PAB6516) staining (2 ug/mL) of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
OSBPL5 polyclonal antibody (Cat # PAB6516) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence
OSBPL5 polyclonal antibody (Cat # PAB6516) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
OSBPL5 polyclonal antibody (Cat # PAB6516) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of OSBPL5.
Immunogen
A synthetic peptide corresponding to human OSBPL5.
Sequence
KEEAFLRRRFSLC
Host
Goat
Theoretical MW (kDa)
98.4, 90.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_065947.1, NP_663613.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (5 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
OSBPL5 polyclonal antibody (Cat # PAB6516) staining (2 ug/mL) of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
OSBPL5 polyclonal antibody (Cat # PAB6516) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
OSBPL5 polyclonal antibody (Cat # PAB6516) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
OSBPL5 polyclonal antibody (Cat # PAB6516) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — OSBPL5
Entrez GeneID
114879Protein Accession#
NP_065947.1;NP_663613.1Gene Name
OSBPL5
Gene Alias
FLJ42929, OBPH1, ORP5
Gene Description
oxysterol binding protein-like 5
Omim ID
606733Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OSBP-related protein 5|OTTHUMP00000013404|OTTHUMP00000013405|oxysterol-binding protein homologue 1|oxysterol-binding protein-like protein 5|oxysterol-binding protein-related protein 5
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Interactome
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Publication Reference
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Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse.
Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T.
Genomics 2002 Dec; 80(6):575.
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Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse.
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