NET1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NET1.
Immunogen
A synthetic peptide corresponding to human NET1.
Sequence
C-DKALSGGKRKETLV
Host
Goat
Theoretical MW (kDa)
67.7, 61.9
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NET1 polyclonal antibody (Cat # PAB6510) staining (2.5 ug/mL) of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NET1
Entrez GeneID
10276Protein Accession#
NP_001040625.1;NP_005854.2Gene Name
NET1
Gene Alias
ARHGEF8, NET1A
Gene Description
neuroepithelial cell transforming 1
Omim ID
606450Gene Ontology
HyperlinkOther Designations
OTTHUMP00000019002|OTTHUMP00000019003|Rho guanine nucleotide exchange factor (GEF) 8|guanine nucleotide regulatory protein (oncogene)|neuroepithelial cell transforming gene 1|neuroepithelioma transforming gene 1|p65 Net1 proto-oncogene protein|small GTP-b
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Interactome
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Disease
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Publication Reference
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Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.
Chan AM, Takai S, Yamada K, Miki T.
Oncogene 1996 Mar; 12(6):1259.
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Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.
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