STXBP1 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of STXBP1.
Immunogen
A synthetic peptide corresponding to C-terminus of human STXBP1.
Sequence
C-SRVSFEDQAPTME
Host
Goat
Theoretical MW (kDa)
68.7
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STXBP1 polyclonal antibody (Cat # PAB6504) staining (0.1 ug/mL) of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
STXBP1 polyclonal antibody (Cat # PAB6504)(5 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STXBP1
Entrez GeneID
6812Protein Accession#
NP_0.03156.1Gene Name
STXBP1
Gene Alias
EIEE4, MUNC18-1, UNC18, hUNC18, rbSec1
Gene Description
syntaxin binding protein 1
Omim ID
602926Gene Ontology
HyperlinkOther Designations
OTTHUMP00000022196|OTTHUMP00000022197|syntaxin-binding protein 1
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Interactomes
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Diseases
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Publication Reference
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Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release.
Swanson DA, Steel JM, Valle D.
Genomics 1998 Mar; 48(3):373.
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Identification and characterization of the human ortholog of rat STXBP1, a protein implicated in vesicle trafficking and neurotransmitter release.
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