SAE1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SAE1.
Immunogen
A synthetic peptide corresponding to human SAE1.
Sequence
MKGNGIVECLGPK
Host
Goat
Theoretical MW (kDa)
38.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SAE1 polyclonal antibody (Cat # PAB6479) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAE1
Entrez GeneID
10055Protein Accession#
NP_005491Gene Name
SAE1
Gene Alias
AOS1, FLJ3091, HSPC140, SUA1
Gene Description
SUMO1 activating enzyme subunit 1
Gene Ontology
HyperlinkOther Designations
SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like protein SUMO-1 activating enzyme
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Interactome
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Pathway
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Publication Reference
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
The Journal of Biological Chemistry 1999 Apr; 274(15):10618.
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
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