PTBP1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PTBP1.
Immunogen
A synthetic peptide corresponding to human PTBP1.
Sequence
DGIVPDIAVGTKR-C
Host
Goat
Theoretical MW (kDa)
59.6, 59, 57.2, 21.9
Reactivity
Human
Specificity
This antibody is expected to recognize all 4 reported isoforms (represented by NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PTBP1 polyclonal antibody (Cat # PAB6476) staining (1 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PTBP1 polyclonal antibody (Cat # PAB6476, 5 ug/mL) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTBP1
Entrez GeneID
5725Protein Accession#
NP_002810.1;NP_114367.1;NP_114368.1;NP_787041.1Gene Name
PTBP1
Gene Alias
HNRNP-I, HNRNPI, HNRPI, MGC10830, MGC8461, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB
Gene Description
polypyrimidine tract binding protein 1
Omim ID
600693Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
RNA-binding protein|heterogeneous nuclear ribonucleoprotein I|heterogeneous nuclear ribonucleoprotein polypeptide I|polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I)|polypyrimidine tract-binding protein 1
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Interactome
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Disease
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Publication Reference
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Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
Charlet-B N, Logan P, Singh G, Cooper TA.
Molecular Cell 2002 Mar; 9(3):649.
Application:IP, Human, HeLa cells.
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Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
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