SIL1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIL1.
Immunogen
A synthetic peptide corresponding to human SIL1.
Sequence
C-ELLGSVNSLLKELR
Host
Goat
Theoretical MW (kDa)
52.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SIL1 polyclonal antibody (Cat # PAB6468) staining (1 ug/mL) of human kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIL1
Entrez GeneID
64374Protein Accession#
NP_071909.1Gene Name
SIL1
Gene Alias
BAP, MSS, ULG5
Gene Description
SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq
Other Designations
BiP-associated protein|SIL1 protein
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Interactome
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Publication Reference
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BAP, a mammalian BiP-associated protein, is a nucleotide exchange factor that regulates the ATPase activity of BiP.
Chung KT, Shen Y, Hendershot LM.
The Journal of Biological Chemistry 2002 Dec; 277(49):47557.
Application:IP, WB-Ce, Human, HepG2, 293T cells.
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BAP, a mammalian BiP-associated protein, is a nucleotide exchange factor that regulates the ATPase activity of BiP.
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