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Last updated: 2017/12/10

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SMARCE1 polyclonal antibody

  • Catalog # : PAB6459
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SMARCE1.
  • Immunogen:
  • A synthetic peptide corresponding to human SMARCE1.
  • Sequence:
  • C-PPTDPIPEDEKKE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 46.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.2-0.6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SMARCE1 polyclonal antibody (Cat # PAB6459) staining (0.2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing SMARCE1 and probed with SMARCE1 polyclonal antibody (Cat # PAB6459) (mock transfection in first lane) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • In paraffin embedded human prostate shows nuclear staining in secretory epithelial cells. SMARCE1 polyclonal antibody (Cat # PAB6459) recommended concentration at 3-5 ug/mL.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6605
  • Gene Name:
  • SMARCE1
  • Gene Alias:
  • BAF57
  • Gene Description:
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
  • Gene Summary:
  • The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq
  • Other Designations:
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1,mammalian chromatin remodeling complex BRG1-associated factor 57
  • Related Disease
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