SMARCE1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMARCE1.
Immunogen
A synthetic peptide corresponding to human SMARCE1.
Sequence
C-PPTDPIPEDEKKE
Host
Goat
Theoretical MW (kDa)
46.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.2-0.6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SMARCE1 polyclonal antibody (Cat # PAB6459) staining (0.2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SMARCE1 and probed with SMARCE1 polyclonal antibody (Cat # PAB6459) (mock transfection in first lane) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
In paraffin embedded human prostate shows nuclear staining in secretory epithelial cells. SMARCE1 polyclonal antibody (Cat # PAB6459) recommended concentration at 3-5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMARCE1
Entrez GeneID
6605Protein Accession#
NP_003070.3Gene Name
SMARCE1
Gene Alias
BAF57
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Omim ID
603111Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq
Other Designations
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1|mammalian chromatin remodeling complex BRG1-associated factor 57
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Interactome
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Disease
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Publication Reference
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Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes.
Wang W, Chi T, Xue Y, Zhou S, Kuo A, Crabtree GR.
PNAS 1998 Jan; 95(2):492.
Application:IP, WB-Ce, WB-Tr, Human, Mouse, Rat, C2F3, C33A, DT, F9, FAO, HeLa, HepG2, Jurkat, PC12, SW13 cells .
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Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes.
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