FBXL12 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FBXL12.
Immunogen
A synthetic peptide corresponding to C-terminus of human FBXL12.
Sequence
RACPKESMDWWM
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FBXL12 polyclonal antibody (Cat # PAB6456) (4 ug/mL) staining of paraffin embedded human lung. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXL12
Entrez GeneID
54850Protein Accession#
NP_060173.1Gene Name
FBXL12
Gene Alias
FLJ20188, Fbl12
Gene Description
F-box and leucine-rich repeat protein 12
Omim ID
609079Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
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Interactome
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Publication Reference
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A family of mammalian F-box proteins.
Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW.
Current Biology 1999 Oct; 9(20):1180.
Application:WB-Tr, Insect cells, Skp1 protein.
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A family of mammalian F-box proteins.
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