FBXL2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FBXL2.
Immunogen
A synthetic peptide corresponding to human FBXL2.
Sequence
VFSNNDEGLINKK-C
Host
Goat
Theoretical MW (kDa)
47.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FBXL2 polyclonal antibody (Cat # PAB6450) staining (0.3 ug/mL) of human brain lysates (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXL2
Entrez GeneID
25827Protein Accession#
NP_0.36289.2Gene Name
FBXL2
Gene Alias
DKFZp564P0622, FBL2, FBL3
Gene Description
F-box and leucine-rich repeat protein 2
Omim ID
605652Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. [provided by RefSeq
Other Designations
F-box protein containing leucine-rich repeats
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Interactome
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Publication Reference
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cDNA cloning and expression analysis of new members of the mammalian F-box protein family.
Ilyin GP, Rialland M, Pigeon C, Guguen-Guillouzo C.
Genomics 2000 Jul; 67(1):40.
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cDNA cloning and expression analysis of new members of the mammalian F-box protein family.
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